Fabrication of biosensors using printing techniques

• Grant number: 11/05742-0
• Support Opportunities: Scholarships abroad - Research
• Start date: October 10, 2011
• End date: September 03, 2012
• Field of knowledge: Physical Sciences and Mathematics - Physics - Condensed Matter Physics
• Principal Investigator: Felippe José Pavinatto
• Grantee: Felippe José Pavinatto
• Host Investigator: Ana Claudia Arias
• Host Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Institution abroad: University of California, Berkeley (UC Berkeley), United States
• Associated research grant: 07/08688-0 - Electronic and optoelectronic polymer devices, AP.TEM

Abstract

Biosensors have been extensively used to precisely detect the concentration of compounds involved in several applications, as in the control of industrial processes, foods, health and biotechnology. Efficient biosensors, in terms of selectivity, stability and detection limit, are already developed in research centers around the world. However, their commercialization is still restricted, mainly due to the lack of cheap and reproducible methods to process biomolecules in large scale. In this project, we propose to study printing techniques applicable to polymeric solutions, as inkjet printing, microcontact-printing and rotogravure, and to adapt such techniques to the printing of solutions of biomolecules, as the enzymes glucose oxidase and tyrosinase. Parameters like solution concentration and viscosity, solvent polarity, surface free energy of the printing surfaces, and composition (plastic membranes will be tested) or superficial treatment of the substrates will be optimized in order to allow the printing of aqueous solutions of the enzymes. Another strategy to be explored is the printing of mixed enzyme-conducting polymer (poly-thiophene, poly-phenylenevinylene, poly-pyrrol) solutions, what allow us to envisage the production of many architectures of field-effect devices. These biosensors have the advantage of combining the recognition and transduction elements, and they can be produced exclusively by printing. The electrical properties of the produced biosensors will be evaluated and used for detection. Such properties will be compared to those obtained from the biosensors already studied in our labs. The responses will also be used as a feedback, and some steps of the printing process could be optimized. The generation of scientific knowledge in physics, chemistry and engineering is expected, and the development of some technological products in the biosensors area is also possible. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PAVINATTO, FELIPPE J.; PASCHOAL, CARLOS W. A.; ARIAS, ANA C.. Printed and flexible biosensor for antioxidants using interdigitated inkjetted electrodes and gravure-deposited active layer. BIOSENSORS & BIOELECTRONICS, v. 67, n. SI, p. 553-559, MAY 15 2015. (11/05742-0)

KHAN, YASSER; PAVINATTO, FELIPPE J.; LIN, MONICA C.; LIAO, AMY; SWISHER, SARAH L.; MANN, KAYLEE; SUBRAMANIAN, VIVEK; MAHARBIZ, MICHEL M.; ARIAS, ANA C.. Inkjet-Printed Flexible Gold Electrode Arrays for Bioelectronic Interfaces. ADVANCED FUNCTIONAL MATERIALS, v. 26, n. 7, p. 1004-1013, FEB 16 2016. (11/05742-0)

PAVINATTO, FELIPPE J.; PASCHOAL, CARLOS W. A.; ARIAS, ANA C.. Printed and flexible biosensor for antioxidants using interdigitated inkjetted electrodes and gravure-deposited active layer. BIOSENSORS & BIOELECTRONICS, v. 67, p. 7-pg., 2015-05-15. (11/05742-0)