Development of a Production and Purification Platform for Bioproducts

• Grant number: 16/05930-4
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2016
• End date: October 31, 2018
• Field of knowledge: Engineering - Chemical Engineering
• Principal Investigator: Angela Faustino Jozala
• Grantee: Angela Faustino Jozala
• Host Institution: Pró-Reitoria Acadêmica. Universidade de Sorocaba (UNISO). Sorocaba , SP, Brazil
• City of the host institution: Sorocaba
• Associated researchers: Denise Grotto ; Luciane Cruz Lopes ; Norberto Aranha ; Valéria de Carvalho Santos Ebinuma

Abstract

Nowadays, the development of bioproducts with high added value, especially when they encourage processes that generate innovative products, applicable to health, sustainable and that bring significant improvements to existing products and processes, have gained much attention. Therefore, there is a trend in researches in the production and application of bioproducts, such as dressings, because they promote patient comfort, especially for pain relief. In this context, the bacterial nanocellulose can be applied as skin dressing for treatment of ulcerative lesions, it relieves pain, protects against bacterial infections and contributed in the process of the tissue regeneration. A promising and innovative alternative to optimize the benefits attributed to bacterial nanocellulose would be its association with polymer composites, such as fibroin; and further, the biomolecules which have antimicrobial activity, antioxidant, among others; in order to overcome their limitations and increase its applications areas. For this reason, the aim of this study it will be to develop and evaluate the properties of a bioproduct created from the combination of nanocellulose with fibroin , further add a biomolecule (bromelain). The bromelain is a proteolytic enzyme used in the food industry and medicine. For this reason, the methodologies were design in three phases, where phase 1 will produce the nanocellulose, produce fibroin; and start the bromelain extraction system by aqueous two phases. Phase 2 will start the biomaterial production, biomolecule stability after extraction. Following for Phase 3, will development the bromelain, after extraction by aqueous two-phase system, incorporation and release in the biomaterial (fibroin and bacterial nanocellulose); the bioproduct mechanical and physicochemical characterization; and biological assays. As a result, we look forward the development a platform for the production and purification of bioproducts, with high properties to apply in wound care. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DOS SANTOS, CAROLINA ALVES; DOS SANTOS, GABRIELA RIBEIRO; SOEIRO, VICTORIA SOARES; DOS SANTOS, JULIA RODRIGUES; REBELO, MARCIA DE ARAUJO; CHAUD, MARCO VINICIUS; GERENUTTI, MARLI; GROTTO, DENISE; PANDIT, RAKSHA; RAI, MAHENDRA; et al. Bacterial nanocellulose membranes combined with nisin: a strategy to prevent microbial growth. Cellulose, v. 25, n. 11, p. 6681-6689, NOV 2018. (16/05930-4)

MALHEIROS, PATRICIA S.; JOZALA, ANGELA F.; PESSOA-JR, ADALBERTO; VILA, MARTA M. D. C.; BALCAO, VICTOR M.; FRANCO, BERNADETTE D. G. M.. Immobilization of antimicrobial peptides from Lactobacillus sakei subsp. sakei 2a in bacterial cellulose: Structural and functional stabilization. FOOD PACKAGING AND SHELF LIFE, v. 17, p. 25-29, SEP 2018. (09/14897-7, 16/05930-4)

VICTÓRIA SOARES SOEIRO; LOUISE LACALENDOLA TUNDISI; VENÂNCIO ALVES AMARAL; FENANDO BATAIN; PRISCILA GAVA MAZZOLA; ELIAS BASILE TAMBOURGI; JOSÉ MARTINS DE OLIVEIRA JÚNIOR; MARCO VINICIUS CHAUD; DENISE GROTTO; NORBERTO ARANHA; et al. Nanocelulose bacteriana e fibroína: produtos naturais para a produção de membranas estruturais. MATERIA-RIO DE JANEIRO, v. 26, n. 4, 2022-01-05. (18/13432-0, 16/05930-4)

ATAIDE, JANAINA ARTEM; DE CARVALHO, NATHALIA MENDES; REBELO, MARCIA DE ARAUJO; CHAUD, MARCO VINICIUS; GROTTO, DENISE; GERENUTTI, MARLI; RAI, MAHENDRA; MAZZOLA, PRISCILA GAVA; JOZALA, ANGELA FAUSTINO. Bacterial Nanocellulose Loaded with Bromelain: Assessment of Antimicrobial, Antioxidant and Physical-Chemical Properties. SCIENTIFIC REPORTS, v. 7, DEC 21 2017. (11/23076-7, 16/05930-4)

FERNANDES, ANA I.; JOZALA, ANGELA F.. Polymers Enhancing Bioavailability in Drug Delivery. PHARMACEUTICS, v. 14, n. 10, p. 7-pg., 2022-10-01. (19/22626-5, 16/05930-4)

OLIVEIRA, THAIS JARDIM; SEGATO, TALITA CRISTINA MENA; MACHADO, GABRIEL PEREIRA; GROTTO, DENISE; JOZALA, ANGELA FAUSTINO. Evolution of Bacterial Cellulose in Cosmetic Applications: An Updated Systematic Review. Molecules, v. 27, n. 23, p. 9-pg., 2022-12-01. (16/05930-4)

DOS SANTOS, GABRIELA RIBEIRO; SOEIRO, VICTORIA SOARES; TALARICO, CAROLINA FERNANDA; ATAIDE, JANAINA ARTEM; LOPES, ANDRE MORENI; MAZZOLA, PRISCILA GAVA; OLIVEIRA, THAIS JARDIM; OLIVEIRA JUNIOR, JOSE MARTINS; GROTTO, DENISE; JOZALA, ANGELA F.. Bacterial Cellulose Membranes as Carriers for Nisin: Incorporation, Antimicrobial Activity, Cytotoxicity and Morphology. POLYMERS, v. 14, n. 17, p. 10-pg., 2022-09-01. (16/05930-4)