Xylella fastidiosa-vector-host plant interaction and approaches for citrus variegated chlorosis and citrus canker control

• Grant number: 13/10957-0
• Support Opportunities: Research Projects - Thematic Grants
• Start date: November 01, 2016
• End date: May 31, 2022
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Alessandra Alves de Souza
• Grantee: Alessandra Alves de Souza
• Host Institution: Instituto Agronômico (IAC). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). Campinas , SP, Brazil
• Pesquisadores principais: Joao Roberto Spotti Lopes
• Associated researchers: Camila Caldana ; Helvecio Della Coletta Filho ; Marco Aurélio Takita ; Marcos Antonio Machado ; Mônica Alonso Cotta
• Associated research grant(s): 17/20487-2 - European Conference on Xylella fastidiosa: finding answers to a global problem, AR.EXT
• Associated scholarship(s): 20/08287-0 - Genetically modified citrus suppressing Diffusible Signaling Factor (DSF): influence on the acquisition and transmission of Xylella fastidiosa by the vector insect and translocation of the rootstock to the scion, BP.PD; 20/04795-1 - Transformation of sweet orange varieties for functional study and / or gene editing for resistance to citrus bacterial diseases, BP.TT; 19/13222-8 - Use of citrus plants for the functional study of genes associated with resistance to citrus variegated chlorosis and citrus canker, BP.TT; + associated scholarships 19/04568-8 - Maintenance and characterization of transgenic citrus elite events with potential field trials., BP.TT; 19/04722-7 - Evaluation of the role of adhesins in biofilm formation by Xylella fastidiosa subsp. pauca on polysaccharide surface, BP.TT; 19/01447-5 - Getting transgenic rootstock: possibility of increasing levels of scion resistance to phytopathogens through the translocation of diffusible molecules, BP.PD; 18/19980-9 - N-acetylcysteine in acquisition and transmission studies of Xylella fastidiosa in citrus, BP.MS; 18/18551-7 - Development and evaluation of Swingle rootstocks transformed with the rpfF gene of Xylella fastidiosa, BP.IC; 18/14356-5 - Acquisition efficiency of Xylella fastidiosa in artificial diets by different sharpshooter species, BP.TT; 17/14398-7 - III-V semiconductor nanowires as new tools for biology investigations, BP.PD; 15/24271-9 - P-N junctions in III-V semiconductor nanowires: a platform for applied studies, BP.DR; 17/25520-8 - Evaluation of the TA system in the inhibition of X. citri PAMPs in copper stress and PTI repression in sweet orange, BP.IC; 18/03868-5 - Use of citrus and model plants for functional study of genes associated with resistance to citrus variegated chlorosis and citrus canker disease, BP.TT; 17/13885-1 - Use of CRISPR/Cas9 in the editing of susceptibility genes of citrus sinensis to Xanthomonas citri subsp. Citri, BP.DR; 17/16142-0 - Translocation of quorum sensing molecules from transgenic rootstocks to sweet orange canopies: effect on resistance against phytopathogens, BP.PD; 16/20629-9 - Obtaining of the rootstock Swingle transformed with rpfF gene of Xylella fastidiosa, BP.IC - associated scholarships

Abstract

Citrus Variegated Chlorosis (CVC), caused by Xylella fastidiosa subsp. pauca bacterium, is one of the most concerning diseases for the Brazilian citriculture. It is responsible for economic damages around 100 million dollars a year in eradication of diseased plants, replanting, and chemical control of the vectors. CVC is a typical Brazilian problem since in other South or Central American countries, where the disease has already been reported, the citrus industry is not as important as it is in Brazil. Therefore it is a role of the research here in Brazil to study and propose alternatives for the control and/or propose a more efficient and sustainable management with reduced production costs. In these 15 years from the X. fastidiosa genome sequencing much basic knowledge was generated, turning it into one of the ten most studied bacteria in the world. However, few of these studies focused on the interactions among the components of this pathosystem, bacterium-plant-vector. This project aims at the integration of groups that work with X. fastidiosa to enlarge and associate the information of the genetic mechanisms that contribute to the adaptation efficiency of the bacterium, the relationship between colonization in the host and disease expression, the molecular interaction with the insect vector, and the genetic responses of the plant that could be used in medium or long term to control the damages caused by CVC in a more sustainable way and with reduced production costs. In addition to X. fastidiosa, the project proposes to expand the studies to Xanthomonas citri subsp. citri, the causal agent of Citrus Canker disease. This disease has become a greater problem in São Paulo orchards, due to recent changes in regulation regarding the control of Citrus Canker. Thus, because of the similarities between the two plant pathogens and common host, some approaches and control strategies will be used for both plant pathogens X. fastidiosa and X. citri. (AU)

Scientific publications

(30)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MITRE, LETICIA KUSTER; TEIXEIRA-SILVA, NATALIA SOUSA; RYBAK, KATARZYNA; MAGALHAES, DIOGO MACIEL; DE SOUZA-NETO, REINALDO RODRIGUES; ROBATZEK, SILKE; ZIPFEL, CYRIL; DE SOUZA, ALESSANDRA ALVES. The Arabidopsis immune receptor EFR increases resistance to the bacterial pathogens Xanthomonas and Xylella in transgenic sweet orange. Plant Biotechnology Journal, JUN 2021. (19/01447-5, 13/01412-0, 14/50880-0, 13/10957-0)

CASERTA, R.; SOUZA-NETO, R. R.; TAKITA, M. A.; LINDOW, S. E.; DE SOUZA, A. A.. Ectopic Expression of Xylella fastidiosa rpfF Conferring Production of Diffusible Signal Factor in Transgenic Tobacco and Citrus Alters Pathogen Behavior and Reduces Disease Severity. MOLECULAR PLANT-MICROBE INTERACTIONS, v. 30, n. 11, p. 866-875, NOV 2017. (08/57909-2, 13/10957-0)

GOMEZ, LAURA M.; TEIXEIRA-SILVA, NATALIA S.; CASERTA, RAQUEL; TAKITA, MARCO A.; MARQUES, MARCIA O. M.; DE SOUZA, ALESSANDRA A.. Overexpression of Citrus reticulata SAMT in Nicotiana tabacum increases MeSA volatilization and decreases Xylella fastidiosa symptoms. PLANTA, v. 252, n. 6, NOV 13 2020. (19/01447-5, 13/10957-0, 17/16142-0, 14/20422-0)

MITRE, LETICIA KUSTER; TEIXEIRA-SILVA, NATALIA SOUSA; RYBAK, KATARZYNA; MAGALHAES, DIOGO MACIEL; DE SOUZA-NETO, REINALDO RODRIGUES; ROBATZEK, SILKE; ZIPFEL, CYRIL; DE SOUZA, ALESSANDRA ALVES. The Arabidopsis immune receptor EFR increases resistance to the bacterial pathogens Xanthomonas and Xylella in transgenic sweet orange. Plant Biotechnology Journal, v. 19, n. 7, p. 1294-1296, JUL 2021. (13/01412-0, 14/50880-0, 19/01447-5, 13/10957-0)

UGARTE, DANIEL; TIZEI, LUIZ H. G.; COTTA, MONICA A.; DUCATI, CATERINA; MIDGLEY, PAUL A.; EGGEMAN, ALEXANDER S.. Analysis of structural distortion in Eshelby twisted InP nanowires by scanning precession electron diffraction. NANO RESEARCH, v. 12, n. 4, p. 939-946, APR 2019. (13/02300-1, 13/10957-0)

CARVALHO, ISIS GABRIELA BARBOSA; MERFA, MARCUS VINICIUS; TEIXEIRA-SILVA, NATALIA SOUSA; MARTINS, PAULA MARIA MOREIRA; TAKITA, MARCO AURELIO; DE SOUZA, ALESSANDRA ALVES. Overexpression of mqsR in Xylella fastidiosa Leads to a Priming Effect of Cells to Copper Stress Tolerance. FRONTIERS IN MICROBIOLOGY, v. 12, SEP 20 2021. (19/01447-5, 14/50880-0, 16/15741-4, 13/10957-0)

MOREIRA, MURILO; HILLENKAMP, MATTHIAS; DIVITINI, GIORGIO; TIZEI, LUIZ H. G.; DUCATI, CATERINA; COTTA, MONICA A.; RODRIGUES, VARLEI; UGARTE, DANIEL. Improving Quantitative EDS Chemical Analysis of Alloy Nanoparticles by PCA Denoising: Part II. Uncertainty Intervals. Microscopy and Microanalysis, v. 28, n. 3, p. 9-pg., 2022-04-18. (14/01045-0, 13/10957-0, 16/12807-4, 07/01722-9, 13/02300-1)

ANBUMANI, SILAMBARASAN; DA SILVA, ALDELIANE M.; ALAFERDOV, ANDREI; DOS SANTOS, MARCOS V. PUYDINGER; CARVALHO, ISIS G. B.; SOUZA E SILVA, MARIANA DE; MOSHKALEV, STANISLAV; CARVALHO, HERNANDES F.; DE SOUZA, ALESSANDRA A.; COTTA, MONICA A.. Physiochemically Distinct Surface Properties of SU-8 Polymer Modulate Bacterial Cell-Surface Holdfast and Colonization. ACS APPLIED BIO MATERIALS, v. 5, n. 10, p. 10-pg., 2022-09-26. (17/14398-7, 13/10957-0, 14/50938-8, 19/07616-3)

ANBUMANI, SILAMBARASAN; DA SILVA, ALDELIANE M.; CARVALHO, ISIS G. B.; FISCHER, EDUARDA REGINA; DE SOUZA E SILVA, MARIANA; VON ZUBEN, ANTONIO AUGUSTO G.; CARVALHO, HERNANDES F.; DE SOUZA, ALESSANDRA A.; JANISSEN, RICHARD; COTTA, MONICA A.. Controlled spatial organization of bacterial growth reveals key role of cell filamentation preceding Xylella fastidiosa biofilm formation. NPJ BIOFILMS AND MICROBIOMES, v. 7, n. 1, DEC 7 2021. (13/10957-0, 19/07616-3, 14/50938-8)

NIZA, BARBARA; MERFA, MARCUS V.; ALENCAR, VALQUIRIA C.; MENEGIDIO, FABIANO B.; NUNES, LUIZ R.; MACHADO, MARCOS A.; TAKITA, MARCO A.; DE SOUZA, ALESSANDRA A.. Draft Genome Sequence of 11399, a Transformable Citrus-Pathogenic Strain of Xylella fastidiosa. GENOME ANNOUNCEMENTS, v. 4, n. 5, p. 2-pg., 2016-09-01. (13/10957-0, 13/17485-7, 11/14307-5, 13/02014-9, 08/57909-2)

MONTEIRO, MONIELLEN P.; HERNANDEZ-MONTELONGO, JACOBO; SAHOO, PRASANA K.; MONTELONGO, ROSAURA HERNANDEZ; DE OLIVEIRA, DOUGLAS S.; PIAZZETA, MARIA H. O.; GARCIA SANDOVAL, JUAN P.; DE SOUZA, ALESSANDRA A.; GOBBI, ANGELO L.; COTTA, MONICA A.. Functionalized microchannels as xylem-mimicking environment: Quantifying X. fastidiosa cell adhesion. BIOPHYSICAL JOURNAL, v. 120, n. 8, p. 1443-1453, APR 20 2021. (10/51748-7, 15/16611-4, 13/10957-0)

PEREIRA, WILLIAN; TAKITA, MARCO; MELOTTO, MAELI; DE SOUZA, ALESSANDRA. Citrus reticulata CrRAP2.2 Transcriptional Factor Shares Similar Functions to the Arabidopsis Homolog and Increases Resistance to Xylella fastidiosa. MOLECULAR PLANT-MICROBE INTERACTIONS, v. 33, n. 3, p. 519-527, MAR 2020. (13/26944-5, 13/10957-0)

MARTINS, PAULA M. M.; WOOD, THOMAS K.; DE SOUZA, ALESSANDRA A.. Persister Cells Form in the Plant Pathogen Xanthomonas citri subsp. citri under Different Stress Conditions. MICROORGANISMS, v. 9, n. 2, FEB 2021. (14/50880-0, 13/10957-0)

PEREIRA, WILLIAN EDUARDO LINO; DE ANDRADE, SHEILA MARIA PEREIRA; DEL PONTE, EMERSON M.; ESTEVES, MARIANA BOSSI; CANALE, MARIA CRISTINA; TAKITA, MARCO AURELIO; COLETTA-FILHO, HELVECIO DELLA; DE SOUZA, ALESSANDRA ALVES. Severity assessment in the Nicotiana tabacum-Xylella fastidiosa subsp. pauca pathosystem: design and interlaboratory validation of a standard area diagram set. ROPICAL PLANT PATHOLOG, v. 45, n. 6, p. 13-pg., 2020-11-09. (11/14629-2, 16/01273-9, 13/10957-0, 14/50880-0)

NIZA, BARBARA; MERFA, MARCUS V.; ALENCAR, VALQUIRIA C.; MENEGIDIO, FABIANO B.; NUNES, LUIZ R.; MACHADO, MARCOS A.; TAKITA, MARCO A.; DE SOUZA, ALESSANDRA A.. Draft Genome Sequence of 11399, a Transformable Citrus-Pathogenic Strain of Xylella fastidiosa. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 4, n. 5, SEP-OCT 2016. (13/02014-9, 11/14307-5, 13/17485-7, 13/10957-0, 08/57909-2)

PEREIRA, WILLIAN EDUARDO LINO; DE ANDRADE, SHEILA MARIA PEREIRA; DEL PONTE, EMERSON M.; ESTEVES, MARIANA BOSSI; CANALE, MARIA CRISTINA; TAKITA, MARCO AURELIO; COLETTA-FILHO, HELVECIO DELLA; DE SOUZA, ALESSANDRA ALVES. Severity assessment in the Nicotiana tabacum-Xylella fastidiosa subsp. pauca pathosystem: design and interlaboratory validation of a standard area diagram set. TROPICAL PLANT PATHOLOGY, v. 45, n. 6, SI, NOV 2020. (14/50880-0, 16/01273-9, 13/10957-0, 11/14629-2)

MOREIRA MARTINS, PAULA MARIA; ANDRADE, MAXUEL DE OLIVEIRA; BENEDETTI, CELSO EDUARDO; DE SOUZA, ALESSANDRA ALVES. Xanthomonas citri subsp. citri: host interaction and control strategies. TROPICAL PLANT PATHOLOGY, v. 45, n. 3, SI, JUN 2020. (13/10957-0, 18/08535-4, 14/50880-0)

VAZ, JULIANA M.; TAKETA, THIAGO B.; HERNANDEZ-MONTELONGO, JACOBO; CHEVALLIER, PASCALE; COTTA, MONICA A.; MANTOVANI, DIEGO; BEPPU, MARISA M.. Antibacterial properties of chitosan-based coatings are affected by spacer-length and molecular weight. Applied Surface Science, v. 445, p. 478-487, JUL 1 2018. (13/14888-3, 10/51748-7, 13/10957-0, 13/05135-1)

DA SILVA, ALDELIANE M.; SAHOO, PRASANA K.; CAVALLI, ALESSANDRO; DE SOUZA, ALESSANDRA A.; BAKKERS, ERIK P. A. M.; CESAR, CARLOS L.; JANISSEN, RICHARD; COTTA, MONICA A.. Nanowire Arrays as Force Sensors with Super-Resolved Localization Position Detection: Application to Optical Measurement of Bacterial Adhesion Forces. SMALL METHODS, v. 2, n. 7, JUL 10 2018. (13/02300-1, 10/51748-7, 13/10957-0)

PICCHI, SIMONE CRISTINA; GRANATO, LAIS MOREIRA; FRANZINI, MARIA JULIA FESTA; ANDRADE, MAXUEL OLIVEIRA; TAKITA, MARCO AURELIO; MACHADO, MARCOS ANTONIO; DE SOUZA, ALESSANDRA ALVES. Modified Monosaccharides Content of Xanthan Gum Impairs Citrus Canker Disease by Affecting the Epiphytic Lifestyle of Xanthomonas citri subsp. citri. MICROORGANISMS, v. 9, n. 6, JUN 2021. (13/10957-0, 17/18570-9, 14/50880-0, 13/01395-9, 19/01901-8)

MARTINS, PAULA M. M.; MERFA, MARCUS V.; TAKITA, MARCO A.; DE SOUZA, ALESSANDRA A.. Persistence in Phytopathogenic Bacteria: Do We Know Enough?. FRONTIERS IN MICROBIOLOGY, v. 9, MAY 25 2018. (16/01273-9, 14/50880-0, 13/10957-0)

GRANATO, LAIS MOREIRA; PICCHI, SIMONE CRISTINA; ANDRADE, MAXUEL DE OLIVEIRA; MARTINS, PAULA MARIA MOREIRA; TAKITA, MARCO AURELIO; MACHADO, MARCOS ANTONIO; DE SOUZA, ALESSANDRA ALVES. The ecnA Antitoxin Is Important Not Only for Human Pathogens: Evidence of Its Role in the Plant Pathogen Xanthomonas citri subsp. citri. Journal of Bacteriology, v. 201, n. 20, OCT 2019. (13/10957-0, 14/50880-0)

MOREIRA MARTINS, PAULA MARIA; ANDRADE, MAXUEL DE OLIVEIRA; BENEDETTI, CELSO EDUARDO; DE SOUZA, ALESSANDRA ALVES. Xanthomonas citri subsp. citri: host interaction and control strategies. ROPICAL PLANT PATHOLOG, v. 45, n. 3, p. 24-pg., 2020-06-08. (13/10957-0, 18/18550-0, 14/50880-0, 18/08535-4, 17/18570-9, 16/01273-9)

CARDOSO, JESSICA L. S.; SOUZA, ALESSANDRA A.; VIEIRA, MARIA LUCIA C.. Molecular basis for host responses to Xanthomonas infection. PLANTA, v. 256, n. 4, p. 21-pg., 2022-10-01. (13/10957-0)

PICCHI, SIMONE CRISTINA; DE SOUZA E SILVA, MARIANA; SALDANHA, LUIZ LEONARDO; FERREIRA, HENRIQUE; TAKITA, MARCO AURELIO; CALDANA, CAMILA; DE SOUZA, ALESSANDRA ALVES. GC-TOF/MS-based metabolomics analysis to investigate the changes driven by N-Acetylcysteine in the plant-pathogen Xanthomonas citri subsp. citri. SCIENTIFIC REPORTS, v. 11, n. 1, JUL 30 2021. (14/50880-0, 13/01395-9, 13/10957-0, 18/10734-5)

MOREIRA, MURILO; HILLENKAMP, MATTHIAS; DIVITINI, GIORGIO; TIZEI, LUIZ H. G.; DUCATI, CATERINA; COTTA, MONICA A.; RODRIGUES, VARLEI; UGARTE, DANIEL. Improving Quantitative EDS Chemical Analysis of Alloy Nanoparticles by PCA Denoising: Part I, Reducing Reconstruction Bias. Microscopy and Microanalysis, JAN 2022. (13/02300-1, 13/10957-0, 16/12807-4, 07/01722-9, 14/01045-0)

MERFA, MARCUS VINICIUS; FISCHER, EDUARDA REGINA; SILVA, MARIANA DE SOUZA E.; FRANCISCO, CAROLINA SARDINHA; DELLA COLETTA-FILHO, HELVECIO; DE SOUZA, ALESSANDRA ALVES. robing the Application of OmpA-Derived Peptides to Disrupt the Acquisition of `Candidatus Liberibacter asiaticus' by Diaphorina citr. PHYTOPATHOLOGY, v. 112, n. 1, p. 163-172, JAN 2022. (13/10957-0)

DE SOUZA-NETO, REINALDO RODRIGUES; BARBOSA CARVALHO, ISIS GABRIELA; MOREIRA MARTINS, PAULA MARIA; PICCHI, SIMONE CRISTINA; TOMAZ, JUAREZ PIRES; CASERTA, RAQUEL; TAKITA, MARCO AURELIO; DE SOUZA, ALESSANDRA ALVES. qsR toxin as a biotechnological tool for plant pathogen bacterial contro. SCIENTIFIC REPORTS, v. 12, n. 1, FEB 18 2022. (17/13885-1, 17/16142-0, 13/10957-0, 16/15741-4, 14/50880-0, 16/01273-9)

PEREIRA, W. E. L.; FERREIRA, C. B.; CASERTA, R.; MELOTTO, M.; DE SOUZA, A. A.. Xylella fastidiosa subsp. pauca and fastidiosa Colonize Arabidopsis Systemically and Induce Anthocyanin Accumulation in Infected Leaves. PHYTOPATHOLOGY, v. 109, n. 2, p. 225-232, FEB 2019. (15/50462-6, 16/01273-9, 13/10957-0)

NASCIMENTO, CESAR AUGUSTO; TEIXEIRA-SILVA, NATALIA SOUSA; CASERTA, RAQUEL; MARQUES, MARCIA ORTIZ MAYO; TAKITA, MARCO AURELIO; DE SOUZA, ALESSANDRA A.. Overexpression of CsSAMT in Citrus sinensis Induces Defense Response and Increases Resistance to Xanthomonas citri subsp. citri. FRONTIERS IN PLANT SCIENCE, v. 13, p. 13-pg., 2022-03-24. (19/01447-5, 17/16142-0, 13/10957-0)