Marine and Antarctic biotechnology: microbial enzymes and their applications

• Grant number: 13/19486-0
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2014
• End date: September 30, 2016
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Lara Durães Sette
• Grantee: Lara Durães Sette
• Host Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil
• Associated researchers: Adalberto Pessoa Junior ; André Rodrigues ; Eleni Gomes ; Fernando Carlos Pagnocca ; Henrique Ferreira ; Maria das Graças de Almeida Felipe ; Mauricio Bacci Junior ; Milene Ferro ; Rafaella Costa Bonugli Santos

Abstract

The microorganisms that inhabit poorly explored and/or extreme ecological niches are potential sources for the discovery of new natural products with specific properties for biotechnological applications. Previous studies related to the Antarctic and marine microbial prospecting revealed the potential of these organisms to the production of active enzymes in salinity conditions or at mild temperatures, which can be applied to various sectors of economic importance, as well as to the degradation of environmental pollutants. Thus, this project aims to continue the studies related to the fungi derived from marine environments from Brazilian coast and terrestrial and marine environments from Antarctic ecosystems, by performing new researches related to the production of enzymes (ligninases, xylanases, lipases, proteases, cellulases and L-asparaginase) and by advancing in the studies related to their characterization, up scaling and technological applications. For that end, the focus of the present study will be filamentous fungi and yeasts that were isolated and selected in projects funded by FAPESP, CNPq and the European Community. These microbial resources are preserved in the research collections associated to the Brazilian Collection of Environment and Industrial Microorganisms (CBMAI) from CPQBA/UNICAMP and to the Central of Microbial Resources from UNESP (CRM-UNESP). The results obtained in this project may derive patents related to the production and application of enzymes in industrial and environmental processes. (AU)

Scientific publications

(27)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GURPILHARES, DANIELA DE BORBA; MOREIRA, TATIANE RODRIGUES; BUENO, JULIA DA LUZ; CINELLI, LEONARDO PAES; MAZZOLA, PRISCILA GAVA; PESSOA, ADALBERTO; SETTE, LARA DURAES. ``Algae's sulfated polysaccharides modifications: Potential use of microbial enzymes{''}. Process Biochemistry, v. 51, n. 8, p. 989-998, AUG 2016. (13/19486-0)

FERNANDES DUARTE, ALYSSON WAGNER; LOPES, ANDRE MORENI; DUTRA MOLINO, JOAO VITOR; PESSOA, ADALBERTO; SETTE, LARA DURAES. Liquid-liquid extraction of lipase produced by psychrotrophic yeast Leucosporidium scottii L117 using aqueous two-phase systems. Separation and Purification Technology, v. 156, n. 2, p. 215-225, DEC 17 2015. (10/17033-0, 13/19486-0, 10/08352-5)

CHAVES, FLAVIANA DA SILVA; BRUMANO, LARISSA PEREIRA; FRANCO MARCELINO, PAULO RICARDO; DA SILVA, SILVIO SILVERIO; SETTE, LARA DURAES; FELIPE, MARIA DAS GRACAS DE ALMEIDA. Biosurfactant production by Antarctic-derived yeasts in sugarcane straw hemicellulosic hydrolysate. BIOMASS CONVERSION AND BIOREFINERY, MAY 2021. (13/19486-0)

FERNANDES DUARTE, ALYSSON WAGNER; BARATO, MARIANA BLANCO; NOBRE, FERNANDO SUZIGAN; POLEZEL, DANILO AUGUSTO; DE OLIVEIRA, TASSIO BRITO; DOS SANTOS, JULIANA APARECIDA; RODRIGUES, ANDRE; SETTE, LARA DURAES. Production of cold-adapted enzymes by filamentous fungi from King George Island, Antarctica. POLAR BIOLOGY, v. 41, n. 12, p. 2511-2521, DEC 2018. (13/19486-0, 16/07957-7)

WENTZEL, LIA COSTA PINTO; INFORSATO, FABIO JOSE; MONTOYA, QUIMI VIDAURRE; ROSSIN, BRUNA GOMES; NASCIMENTO, NADIA REGINA; RODRIGUES, ANDRE; SETTE, LARA DURAES. Fungi from Admiralty Bay (King George Island, Antarctica) Soils and Marine Sediments. MICROBIAL ECOLOGY, v. 77, n. 1, p. 12-24, JAN 2019. (13/19486-0, 16/07957-7)

LARIO, LUCIANA DANIELA; CHAUD, LUCIANA; ALMEIDA, MARIA DAS GRACAS; CONVERTI, ATTILIO; DURAES SETTE, LARA; PESSOA, ADALBERTO. Production, purification, and characterization of an extracellular acid protease from the marine Antarctic yeast Rhodotorula mucilaginosa L7. FUNGAL BIOLOGY, v. 119, n. 11, p. 1129-1136, NOV 2015. (13/19486-0, 12/23726-4)

BONUGLI-SANTOS, RAFAELLA C.; DOS SANTOS VASCONCELOS, MARIA R.; PASSARINI, MICHEL R. Z.; VIEIRA, GABRIELA A. L.; LOPES, VIVIANE C. P.; MAINARDI, PEDRO H.; DOS SANTOS, JULIANA A.; DUARTE, LIDIA DE AZEVEDO; OTERO, IGOR V. R.; DA SILVA YOSHIDA, ALINE M.; et al. Marine-derived fungi: diversity of enzymes and biotechnological applications. FRONTIERS IN MICROBIOLOGY, v. 6, APR 10 2015. (13/00286-1, 08/06720-7, 13/12505-0, 13/08617-7, 11/18769-3, 09/18399-1, 13/19486-0, 12/12622-3)

LICURSI VIEIRA, GABRIELA ALVES; CABRAL, LUCELIA; RAMOS OTERO, IGOR VINICIUS; FERRO, MILENE; DE FARIA, ADRIANO UEMURA; DE OLIVEIRA, VALERIA MAIA; BACCI, MAURICIO; SETTE, LARA DURAES. Marine associated microbial consortium applied to RBBR textile dye detoxification and decolorization: Combined approach and metatranscriptomic analysis. Chemosphere, v. 267, MAR 2021. (13/19486-0, 18/12098-9, 16/07957-7)

GURPILHARES, DANIELA DE BORBA; MOREIRA, TATIANE RODRIGUES; BUENO, JULIA DA LUZ; CINELLI, LEONARDO PAES; MAZZOLA, PRISCILA GAVA; PESSOA, ADALBERTO; SETTE, LARA DURAES. "Algae's sulfated polysaccharides modifications: Potential use of microbial enzymes". Process Biochemistry, v. 51, n. 8, p. 10-pg., 2016-08-01. (13/19486-0)

VIEIRA, GABRIELA A. L.; MAGRINI, MARIANA JUVENTINA; BONUGLI-SANTOS, RAFAELLA C.; RODRIGUES, MARILI V. N.; SETTE, LARA D.. Polycyclic aromatic hydrocarbons degradation by marine-derived basidiomycetes: optimization of the degradation process. Brazilian Journal of Microbiology, v. 49, n. 4, p. 749-756, OCT-DEC 2018. (13/19486-0, 12/12622-3, 16/07957-7, 10/50190-2)

GIOVANELLA, PATRICIA; DUARTE, LIDIA DE AZEVEDO; KITA, DANIELA MAYUMI; DE OLIVEIRA, VALERIA MAIA; SETTE, LARA DURAES. Effect of biostimulation and bioaugmentation on hydrocarbon degradation and detoxification of diesel-contaminated soil: a microcosm study. JOURNAL OF MICROBIOLOGY, v. 59, n. 7, p. 634-643, JUL 2021. (13/19486-0, 16/17488-4, 14/13205-2)

BUENO, J. L.; SANTOS, P. A. D.; DA SILVA, R. R.; MOGUEL, I. S.; PESSOA, JR., A.; VIANNA, M. V.; PAGNOCCA, F. C.; SETTE, L. D.; GURPILHARES, D. B.. Biosurfactant production by yeasts from different types of soil of the South Shetland Islands (Maritime Antarctica). Journal of Applied Microbiology, v. 126, n. 5, p. 1402-1413, MAY 2019. (13/19486-0)

FERNANDES DUARTE, ALYSSON WAGNER; BONUGLI-SANTOS, RAFAELLA COSTA; FERRAREZI DUARTE, ANA LUCIA; GOMES, ELENI; SETTE, LARA DURAES. Statistical experimental design applied to extracellular lipase production by the marine Antarctic yeast Leucosporidium scottii CRM 728. BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, v. 32, MAR 2021. (13/19486-0, 10/08352-5)

FERNANDES DUARTE, ALYSSON WAGNER; ZAMBRANO PASSARINI, MICHEL RODRIGO; DELFORNO, TIAGO PALLADINO; PELLIZZARI, FRANCIANE MARIA; ZECCHIN CIPRO, CAIO VINICIUS; MONTONE, ROSALINDA CARMELA; PETRY, MARIA VIRGINIA; PUTZKE, JAIR; ROSA, LUIZ HENRIQUE; SETTE, LARA DURAES. Yeasts from macroalgae and lichens that inhabit the South Shetland Islands, Antarctica. ENVIRONMENTAL MICROBIOLOGY REPORTS, v. 8, n. 5, SI, p. 874-885, OCT 2016. (10/17033-0, 13/19486-0, 10/08352-5)

VASCONCELOS, MARIA R. S.; VIEIRA, GABRIELA A. L.; OTERO, IGOR V. R.; BONUGLI-SANTOS, RAFAELLA C.; RODRIGUES, MARILI V. N.; REHDER, VERA L. G.; FERRO, MILENE; BOAVENTURA, SINESIO; BACCI, JR., MAURICIO; SETTE, LARA D.. Pyrene degradation by marine-derived ascomycete: process optimization, toxicity, and metabolic analyses. Environmental Science and Pollution Research, v. 26, n. 12, SI, p. 12412-12424, APR 2019. (13/19486-0, 16/07957-7)

BRENELLI, LIVIA B.; PERSINOTI, GABRIELA F.; FRANCO CAIRO, JOAO PAULO L.; LIBERATO, MARCELO V.; GONCALVES, THIAGO AUGUSTO; OTERO, IGOR V. R.; MAINARDI, PEDRO H.; FELBY, CLAUS; SETTE, LARA D.; SQUINA, FABIO M.. Novel redox-active enzymes for ligninolytic applications revealed from multiomics analyses of Peniophora sp. CBMAI 1063, a laccase hyper-producer strain. SCIENTIFIC REPORTS, v. 9, NOV 26 2019. (13/03061-0, 13/19486-0, 15/07008-2, 14/04105-4, 16/09950-0, 15/06971-3, 15/50590-4, 17/16089-1)

LARIO, LUCIANA DANIELA; PELLEGRINI MALPIEDI, LUCIANA; BRANDAO PEREIRA, JORGE FERNANDO; SETTE, LARA DURAES; PESSOA-JUNIOR, ADALBERTO. Liquid-liquid extraction of protease from cold-adapted yeast Rhodotorula mucilaginosa L7 using biocompatible and biodegradable aqueous two-phase systems. SEPARATION SCIENCE AND TECHNOLOGY, v. 51, n. 1, p. 57-67, JAN 2 2016. (13/19486-0, 11/20521-0, 12/23726-4)

MAINARDI, PEDRO H.; FEITOSA, VALKER A.; BRENELLI DE PAIVA, LIVIA B.; BONUGLI-SANTOS, RAFAELLA C.; SQUINA, FABIO M.; PESSOA, JR., ADALBERTO; SETTE, LARA D.. Laccase production in bioreactor scale under saline condition by the marine-derived basidiomycete Peniophora sp CBMAI 1063. FUNGAL BIOLOGY, v. 122, n. 5, p. 302-309, MAY 2018. (14/06923-6, 13/12505-0, 13/03061-0, 16/07957-7, 13/19486-0)

RAMOS OTERO, IGOR VINICIUS; FERRO, MILENE; BACCI, JR., MAURICIO; FERREIRA, HENRIQUE; SETTE, LARA DURAES. De novo transcriptome assembly: a new laccase multigene family from the marine-derived basidiomycete Peniophora sp CBMAI 1063. AMB EXPRESS, v. 7, DEC 20 2017. (14/17950-4, 13/19486-0, 16/07957-7)

DOS SANTOS, JULIANA A.; VIEIRA, JULIANA M. F.; VIDEIRA, ALEXANDRE; MEIRELLES, LUCAS A.; RODRIGUES, ANDRE; TANIWAKI, MARTA H.; SETTE, LARA D.. Marine-derived fungus Aspergillus cf. tubingensis LAMAI 31: a new genetic resource for xylanase production. AMB EXPRESS, v. 6, MAR 24 2016. (11/16765-0, 10/17033-0, 13/19486-0, 13/08338-0)

LOPES, ANDRE MORENI; RIBEIRO, ARTUR; DE OLIVEIRA, MARCOS ANTONIO; DE SOUZA-MOTTA, CRISTINA MARIA; MAGALHAES, PEROLA DE OLIVEIRA; FARIAS AVENDANO, JORGE GONZALO; MAZZOLA, PRISCILA GAVA; RANGEL-YAGUI, CARLOTA DE OLIVEIRA. Therapeutic l-asparaginase: upstream, downstream and beyond. CRITICAL REVIEWS IN BIOTECHNOLOGY, v. 37, n. 1, p. 82-99, 2017. (13/08617-7, 13/19486-0)

ZAMBRANO PASSARINI, MICHEL RODRIGO; OTTONI, CRISTIANE ANGELICA; SANTOS, CLEDIR; LIMA, NELSON; SETTE, LARA DURAES. Induction, expression and characterisation of laccase genes from the marine-derived fungal strains Nigrospora sp CBMAI 1328 and Arthopyrenia sp CBMAI 1330. AMB EXPRESS, v. 5, MAR 11 2015. (08/06720-7, 13/19486-0, 10/50190-2)

MACHADO, SUELLEN; FEITOSA, VALKER; PILLACA-PULLO, OMAR; LARIO, LUCIANA; SETTE, LARA; PESSOA JR, ADALBERTO; ALVES, HARLEY. Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor. BIOENGINEERING-BASEL, v. 9, n. 11, p. 13-pg., 2022-11-01. (10/17033-0, 12/23726-4, 13/19486-0, 16/07957-7)

CHAVES, FLAVIANA DA SILVA; BRUMANO, LARISSA PEREIRA; FRANCO MARCELINO, PAULO RICARDO; DA SILVA, SILVIO SILVERIO; SETTE, LARA DURAES; FELIPE, MARIA DAS GRACAS DE ALMEIDA. Biosurfactant production by Antarctic-derived yeasts in sugarcane straw hemicellulosic hydrolysate. BIOMASS CONVERSION AND BIOREFINERY, v. 13, n. 6, p. 11-pg., 2021-05-31. (13/19486-0)

VASCONCELOS, MARIA R. S.; VIEIRA, GABRIELA A. L.; OTERO, IGOR V. R.; BONUGLI-SANTOS, RAFAELLA C.; RODRIGUES, MARILI V. N.; REHDER, VERA L. G.; FERRO, MILENE; BOAVENTURA, SINESIO; BACCI, MAURICIO, JR.; SETTE, LARA D.. Pyrene degradation by marine-derived ascomycete: process optimization, toxicity, and metabolic analyses. Environmental Science and Pollution Research, v. 26, n. 12, p. 13-pg., 2019-04-01. (13/19486-0, 16/07957-7)

FERNANDES DUARTE, ALYSSON WAGNER; ZAMBRANO PASSARINI, MICHEL RODRIGO; DELFORNO, TIAGO PALLADINO; PELLIZZARI, FRANCIANE MARIA; ZECCHIN CIPRO, CAIO VINICIUS; MONTONE, ROSALINDA CARMELA; PETRY, MARIA VIRGINIA; PUTZKE, JAIR; ROSA, LUIZ HENRIQUE; SETTE, LARA DURAES. Yeasts from macroalgae and lichens that inhabit the South Shetland Islands, Antarctica. ENVIRONMENTAL MICROBIOLOGY REPORTS, v. 8, n. 5, p. 12-pg., 2016-10-01. (13/19486-0, 10/08352-5, 10/17033-0)

FERNANDES DUARTE, ALYSSON WAGNER; LOPES, ANDRE MORENI; DUTRA MOLINO, JOAO VITOR; PESSOA, ADALBERTO; SETTE, LARA DURAES. Liquid-liquid extraction of lipase produced by psychrotrophic yeast Leucosporidium scottii L117 using aqueous two-phase systems. Separation and Purification Technology, v. 156, p. 11-pg., 2015-12-17. (13/19486-0, 10/08352-5, 10/17033-0)