Molecular approaches to study the relationship between ethylene and sugarcane ripening

• Grant number: 13/15576-5
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
• Start date: March 01, 2014
• End date: February 28, 2019
• Field of knowledge: Biological Sciences - Genetics - Plant Genetics
• Principal Investigator: Marcelo Menossi Teixeira
• Grantee: Marcelo Menossi Teixeira
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated researchers: Ana Maria Magalhaes Andrade Lagoa ; Eduardo Caruso Machado ; Giuseppina Pace Pereira Lima ; Lucia Mattiello ; Marcelo de Almeida Silva ; Rafael Vasconcelos Ribeiro
• Associated scholarship(s): 18/10595-5 - Dissection of the heat stress response in Saccharum spp and in vitro study of the chloroplast HSP70-CLPB-sHSPs disaggregation machinery, BP.PD; 15/23789-4 - EVALUATION OF THE ROLE OF GENES ENCODING ACC SINTHASE IN THE DEVELOPMENT OF SUGARCANE PLANTS, BP.PD; 14/10003-0 - Cross-talk between brassinosteroid and ethylene in sugarcane ripening: regulation of ACC synthase via brassinosteroid, BP.PD; 14/15172-4 - Transcription factors controlling ACC synthase expression in sugarcane (Saccharum spp.), BP.DD

Abstract

Sugarcane (Saccharum spp.) is one of the most important raw materials for Brazilian economy, unique in its ability to accumulate large amounts of sucrose in the stalk. The ripening stage is crucial in agronomic terms, influencing harvesting season, milling operations, and very importantly sucrose yield itself. Despite sugarcane being an excellent model for studying source-sink relationship - synthesis of sucrose in the leaves (source) and accumulation in the internodes (sink) - little is known about the molecular hormonal and developmental regulation of this process. Several plant growth regulators or ripeners, analogous to plant hormones, like ethylene, are commonly applied to sugarcane fields to increase sucrose accumulation, accelerate ripening or inhibit flowering. Ethylene is also identified as the key internal cue that regulates the transition from active vegetative growth to cane maturation (and ripening) phase. With ethylene being one of the most commercially successful hormones used for manipulating sugarcane ripening, and that it is a natural primer for cane maturation, we aim to understand its synthesis and action in relation to sugar accumulation and ripening regulation, and how it can be manipulated to increase sugar yield and crop productivity. We investigate this topic by three different but complementary approaches: (i) identify the key (regulatory) genes (via transcriptome analysis) that are modulated by ethylene during sugarcane ripening and understand how they regulate sucrose accumulation and plant growth; (ii) identify promoters and their regulators of genes encoding the enzyme 1-aminocyclopropane-1-carboxylic acid synthase (ACS), the regulatory enzyme in the biosynthesis of ethylene, to gain molecular insight into the regulation of ethylene in relation to cane ripening; and (iii) characterize functionally the sugarcane gene encoding the DELLA protein, the main integrator of ethylene and gibberellin actions and plant growth and development, and evaluate its role in source-sink regulation using transgenic sugarcane plants with its up- and down-regulation. These studies will expand our understanding of the physiological, molecular and developmental mechanisms involved in sucrose accumulation, contributing to future breeding programs and biotechnological improvement of sugarcane. (AU)

Scientific publications

(11)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BERNARDES, WILLIAN SOUZA; MENOSSI, MARCELO. Plant 3' Regulatory Regions From mRNA-Encoding Genes and Their Uses to Modulate Expression. FRONTIERS IN PLANT SCIENCE, v. 11, AUG 14 2020. (13/15576-5, 14/50884-5)

PERES, ANA LAURA G. L.; SOARES, JOSE SERGIO; TAVARES, RAFAEL G.; RIGHETTO, GERMANNA; ZULLO, MARCO A. T.; MANDAVA, N. BHUSHAN; MENOSSI, MARCELO. Brassinosteroids, the Sixth Class of Phytohormones: A Molecular View from the Discovery to Hormonal Interactions in Plant Development and Stress Adaptation. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 20, n. 2, p. 33-pg., 2019-01-02. (13/15576-5)

GUIDELLI, GIOVANNA, V; MATTIELLO, LUCIA; GALLINARI, RAFAEL H.; DE LUCCA, PAULO CEZAR; MENOSSI, MARCELO. pGVG: a new Gateway-compatible vector for transformation of sugarcane and other monocot crops. GENETICS AND MOLECULAR BIOLOGY, v. 41, n. 2, p. 5-pg., 2018-04-01. (15/08659-7, 13/15576-5, 15/23789-4, 14/26521-0)

GUIDELLI, V, GIOVANNA; MATTIELLO, LUCIA; GALLINARI, RAFAEL H.; DE LUCCA, PAULO CEZAR; MENOSSI, MARCELO. pGVG: a new Gateway-compatible vector for transformation of sugarcane and other monocot crops. GENETICS AND MOLECULAR BIOLOGY, v. 41, n. 2, p. 450-454, APR-JUN 2018. (15/08659-7, 15/23789-4, 13/15576-5, 14/26521-0)

PERES, ANA LAURA G. L.; SOARES, JOSE SERGIO; TAVARES, RAFAEL G.; RIGHETTO, GERMANNA; ZULLO, MARCO A. T.; MANDAVA, N. BHUSHAN; MENOSSI, MARCELO. Brassinosteroids, the Sixth Class of Phytohormones: A Molecular View from the Discovery to Hormonal Interactions in Plant Development and Stress Adaptation. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 20, n. 2, JAN 2 2019. (13/15576-5)

CUNHA, CAMILA P.; ROBERTO, GUILHERME G.; VICENTINI, RENATO; LEMBKE, CAROLINA G.; SOUZA, GLAUCIA M.; RIBEIRO, RAFAEL V.; MACHADO, EDUARDO C.; LAGOA, ANA M. M. A.; MENOSSI, MARCELO. Ethylene-induced transcriptional and hormonal responses at the onset of sugarcane ripening. SCIENTIFIC REPORTS, v. 7, MAR 7 2017. (08/57495-3, 08/54201-9, 12/13920-8, 13/15576-5, 08/52146-0)

MODOLO, DIEGO G.; HORN, CYNTHIA S.; SOARES, JOSE S. M.; YUNES, JOSE A.; LIMA, LEILA M.; DE SOUSA, SYLVIA M.; MENOSSI, MARCELO. Transgenic Nicotiana tabacum seeds expressing the Mycobacterium tuberculosis Alanine- and Proline-rich antigen. AMB EXPRESS, v. 8, OCT 31 2018. (13/15576-5)

ALESSIO, VALTER MIOTTO; CAVACANA, NATALE; DE BARROS DANTAS, LUIZA LANE; LEE, NAYOUNG; HOTTA, CARLOS TAKESHI; IMAIZUMI, TAKATO; MENOSSI, MARCELO. The FBH family of bHLH transcription factors controls ACC synthase expression in sugarcane. Journal of Experimental Botany, v. 69, n. 10, p. 2511-2525, APR 27 2018. (13/15576-5, 15/06260-0, 13/07914-8)

TAVARES, RAFAEL GARCIA; LAKSHMANAN, PRAKASH; PEITER, EDGAR; O'CONNELL, ANTHONY; CALDANA, CAMILA; VICENTINI, RENATO; SOARES, JOSE SERGIO; MENOSSI, MARCELO. ScGAI is a key regulator of culm development in sugarcane. Journal of Experimental Botany, v. 69, n. 16, p. 3823-3837, JUL 20 2018. (13/15576-5, 14/15458-5, 12/06877-9)

COTRIM, CAMILA A.; SOARES, JOSE SERGIO M.; KOBE, BOSTJAN; MENOSSI, MARCELO. Crystal structure and insights into the oligomeric state of UDP-glucose pyrophosphorylase from sugarcane. PLoS One, v. 13, n. 3, MAR 1 2018. (13/15576-5, 08/06767-3)

GARCIA LEME PERES, ANA LAURA; DELLA COLETTA, RAFAEL; SOARES, JOSE SERGIO; MENOSSI, MARCELO. Gene Silencing Using Artificial miRNA in Sugarcane. TROPICAL PLANT BIOLOGY, v. 15, n. 2, p. 10-pg., 2022-03-25. (13/15576-5, 14/50884-5)

Filed patent(s) as a result of this research project