| Texto completo | |
| Autor(es): |
Longato, Giovanna Barbarini
[1, 2]
;
Fiorito, Giovanna Francisco
[1]
;
Vendramini-Costa, Debora Barbosa
[1]
;
de Oliveira Sousa, Ilza Maria
[3]
;
Tinti, Sirlene Valerio
[1]
;
Tasca Gois Ruiz, Ana Lucia
[1]
;
Vitalino de Almeida, Sinara Monica
[4, 5, 6]
;
Ribeiro Padilha, Rafael Jose
[4, 5]
;
Foglio, Mary Ann
[3]
;
de Carvalho, Joao Ernesto
[1, 7]
Número total de Autores: 10
|
| Afiliação do(s) autor(es): | [1] Univ Estadual Campinas, Ctr Pluridisciplinar Pesquisas Quim Biol & Agr CP, Div Farmacol & Toxicol, BR-13083970 Campinas, SP - Brazil
[2] Univ Estadual Campinas UNICAMP, Programa Posgrad Biol Celular & Estrutural, Inst Biol, BR-13083872 Campinas, SP - Brazil
[3] Univ Estadual Campinas, CPQBA, Div Fitoquim, BR-13083970 Campinas, SP - Brazil
[4] Univ Fed Pernambuco UFPE, LIKA, BR-50670901 Recife, PE - Brazil
[5] Univ Fed Pernambuco UFPE, Dept Bioquim, BR-50670901 Recife, PE - Brazil
[6] Univ Pernambuco UPE, Fac Ciencias Educ & Tecnol Garanhuns FACETEG, BR-55290000 Garanhuns, PE - Brazil
[7] Univ Estadual Campinas, Fac Ciencias Farmaceut, BR-13083872 Campinas, SP - Brazil
Número total de Afiliações: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | TOXICOLOGY IN VITRO; v. 29, n. 5, p. 1026-1033, AUG 2015. |
| Citações Web of Science: | 12 |
| Resumo | |
Natural products remain an important source of new drugs, including anticancer drugs. Recently, our group reported the anticancer activity of eupomatenoid-5 (eup-5), a neolignan isolated from Piper regnellii (Miq.) C. DC. var. regnellii leaves. In vitro studies demonstrated that MCF-7 (breast) and 786-0 (kidney) were among the cancer cell lines most sensitive to eup-5 treatment. The current results demonstrate that mitochondrial membrane depolarization and generation of reactive oxygen species are implicated in eup-5-mediated cytotoxic effects on these cancer cells lines. In MCF-7 cells, eup-5 led to phosphatidylserine externalization and caspase activation, whereas the same did not occur in 786-0 cells. Scanning electron microscopy revealed a reduction of microvilli density, as well as cell morphology alterations. Moreover, treated MCF-7 cells exhibited well-characterized apoptosis alterations, while treated 786-0 cells exhibited characteristics of programmed necroptosis process. These findings support the possibility that different mechanisms may be targeted by eup-5 in cell death response. (C) 2015 Elsevier Ltd. All rights reserved. (AU) | |
| Processo FAPESP: | 10/50232-7 - Atividade anticancer e mecanismo de acao de compostos isolados das folhas de piper regnellii (miq.) c. dc. var. regnellii |
| Beneficiário: | Giovanna Barbarini Longato |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |