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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Expression of genes from the lignin synthesis pathway in guineagrass genotypes differing in cell-wall digestibility

Texto completo
Stabile, S. S. ; Bodini, A. P. ; Jank, L. [1] ; Renno, F. P. ; Santos, M. V. ; Silva, L. F. P. [2]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Embrapa Beef Cattle, Campo Grande, MS - Brazil
[2] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Anim Nutr & Prod, BR-13635900 Sao Paulo - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: GRASS AND FORAGE SCIENCE; v. 67, n. 1, p. 43-54, MAR 2012.
Citações Web of Science: 4

Rapid decline in cell-wall digestibility hinders efficient use of warm-season grasses. The objective of this study was to identify genes whose expressions are related to the slope of decline in cell-wall digestibility. Eleven guineagrass genotypes were harvested at three ages and classified according to fibre digestibility. Extreme genotypes were separated into groups with either FAST or SLOW decline in fibre digestibility. Expression of transcripts from six genes from the lignin synthesis pathway was quantified by real-time PCR. Fast decline in fibre digestibility was associated with higher DM yield after 90 d of regrowth. Apart from lower fibre digestibility and higher lignin content for the FAST group, there were no other differences between the two groups for the chemical composition of stems and leaves. Maturity affected differently the expression of two of the six genes, cinnamate 4-hydroxylase and caffeoyl-CoA O-methyltransferase (C4H and CCoAOMT). Genotypes with fast decline in fibre digestibility had greater increase in the expression of C4H and CCoAOMT from 30 to 60 d of regrowth, than genotypes with slower decline. Expression of C4H and CCoAOMT appears to be related to the decline in cell-wall digestibility with advance in maturity of guineagrass. (AU)