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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Zika detection: comparison of methodologies

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Autor(es):
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Colombo, Tatiana Elias [1] ; Bernardes Terzian, Ana Carolina [1] ; Araujo Junior, Joao Pessoa [2] ; Parreira, Ricardo [3] ; Sotello Cabrer, Eliana Marcia [1] ; Penha dos Santos, Izalco Nuremberg [4] ; Negri Reis, Andreia Francesli [4] ; Costa, Fabiana Rodrigues [4] ; Arao Antonio Cruz, Lilian Elisa [4] ; Rombola, Patricia Lopes [4] ; Nogueira, Mauricio Lacerda [1]
Número total de Autores: 11
Afiliação do(s) autor(es):
[1] Fac Med Sao Jose do Rio Preto FAMERP, Sao Jose Do Rio Preto, SP - Brazil
[2] Univ Estadual Paulista, Inst Biociencias Botucatu, Lab Virol, Botucatu, SP - Brazil
[3] Univ Nova Lisboa, GHTM, Lisbon - Portugal
[4] Prefeitura Sao Jose do Rio Preto, Dept Vigilancia Saude, Sao Jose Do Rio Preto, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: Brazilian Journal of Microbiology; v. 49, n. 1, p. 144-147, JAN-MAR 2018.
Citações Web of Science: 0
Resumo

Many countries in the Americas have detected local transmission of multiple arboviruses that cause febrile illnesses. Therefore, laboratory testing has become an important tool for confirming the etiology of these diseases. The present study aimed to compare the sensitivity and specificity of three different Zika virus detection assays. One hundred serum samples from patients presenting with acute febrile symptoms were tested using a previously reported TaqMan (R) RT-qPCR assay. We used a SYBR (R) Green RT-qPCR and a conventional PCR methodologies to compare the results. Of the samples that were determined to be negative by the TaqMan (R) RT-qPCR assay, 100% (Kappa = 0.670) were also found to be negative by SYBR (R) Green RT-qPCR based on Tm comparison; however, 14% (Kappa = 0.035) were found to be positive by conventional PCR followed by agarose gel electrophoresis. The differences between the ZIKV strains circulating worldwide and the low viremia period can compromise diagnostic accuracy and thereby the accuracy of outbreak data. Therefore, improved assays are required to improve the diagnosis and surveillance of arbovirus. (C) 2017 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. (AU)

Processo FAPESP: 13/21719-3 - Estudo epidemiológico da dengue (sorotipos 1 a 4) em coorte prospectiva de São José do Rio Preto, São Paulo, Brasil, durante 2014 a 2018
Beneficiário:Maurício Lacerda Nogueira
Linha de fomento: Auxílio à Pesquisa - Temático