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Several questions have been raised about the best technique for cultivation of limbal epithelial cells ex vivo. Variables such as culture medium, co-culture with feeder layer, substrate (scaffold) for culture must be well studied because they may influence survival and the maintenance of stemness of the cells to be transplanted. This study has been conducted to evaluate the effectiveness of different culture media on growth, proliferation, apoptosis and differentiation of limbal epithelial cells cultivated ex vivo. Corneal rims from different donors will have their posterior stroma removed and will be divided into 12 fragments and cultured in three different culture media: SHEM, KSFM, Epilife. The epithelial cell cultures were submitted to analysis of growth and epithelial migration; immunocytochemistry for ABCG2, p63, Ki67, CK3 and VMT; RT-PCR; and cell viability test with Hoechst. All results will be statistically compared. (AU)


Ferromagnetic order in itinerant electron systems remains a major challenge in the theory of strongly correlated electron systems. Recent interest in this problem has been motivated by experiments with new compounds and with systems of cold atoms in optical traps. In this project, we propose to study properties of one-dimensional systems with long-range magnetic order (ferromagnetic Luttinger liquids) using analytical quantum field theory techniques. We intend to investigate the effect of the coupling between charge and spin on correlation functions, distinguishing between systems with discrete Ising-type spin symmetry and SU(2) symmetry. (AU)

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