Genetic structure and mating system of annatto (Bixa orellana L.) populations from the Brazilian Amazon and Central Brazil using microsatellite markers

• Grant number: 13/08884-5
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: July 01, 2013
• End date: August 01, 2017
• Field of knowledge: Biological Sciences - Genetics - Plant Genetics
• Principal Investigator: Elizabeth Ann Veasey
• Grantee: Gabriel Dequigiovanni
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated scholarship(s): 16/05912-6 - Genetic diversity and phylogeography of annatto (Bixa orellana L.) from Brazilian Amazon, BE.EP.DR

Abstract

Annatto (Bixa orellana L.) is a tropical crop indigenous to America, probably to the Amazon region. This species receives great attention due to the natural red and yellow dyes, extracted from its seeds used in the pharmaceutical, textile, dairy, food, beverage, paints and cosmetics industries. These dyes extracted from annatto are among the few allowed by the World Health Organization (WHO), because they are not toxic and do not alter food taste. Although annatto is an important source of natural dyes, there is almost no research assessing the genetic variability within this species and investigating how this variability is distributed in the Brazilian territory. In our study, we aimed to characterize the level and distribution of genetic diversity of local varieties of annatto collected in the Brazilian Amazon and Central Brazil. The data obtained will allow us to detect possible diversity centers or the center of domestication of this species, and also how the genetic diversity of this crop is structured. In addition, we aim to determine the species reproductive system through progeny tests using microsatellite markers, studying the mating system at the hierarchical level of fruits and individuals from wild and cultivated populations of annatto. We aim to verify whether there are differences regarding the reproduction mode among wild and cultivated populations. The data obtained in this project aims to contribute to breeding programs of annatto in Brazil. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DEQUIGIOVANI, GABRIEL; FERREYRA RAMOS, SANTIAGO LINORIO; ALVES-PEREIRA, ALESSANDRO; FABRI, ELIANE GOMES; NOGUEIRA CARVALHO, PAULO ROBERTO; DA SILVA, MARTA GOMES; VILELA NOGUEIRA ABDO, MARIA TERESA; MELLO MARTINS, ANTONIO LUCIO; CLEMENT, CHARLES ROLAND; VEASEY, ELIZABETH ANN. Genetic diversity and structure in a major Brazilian annatto (Bixa orellana) germplasm bank revealed by microsatellites and phytochemical compounds. Genetic Resources and Crop Evolution, v. 64, n. 7, p. 1775-1788, OCT 2017. (11/10357-8, 12/08307-5, 11/05243-3, 13/08884-5)

DEQUIGIOVANNI, GABRIEL; FERREYRA RAMOS, SANTIAGO LINORIO; GOMES LOPES, MARIA TERESA; CLEMENT, CHARLES ROLAND; RODRIGUES, DORIANE PICANCO; FABRI, ELIANE GOMES; ZUCCHI, MARIA IMACULADA; VEASEY, ELIZABETH ANN. New microsatellite loci for annatto (Bixa orellana), a source of natural dyes from Brazilian Amazonia. Crop Breeding and Applied Biotechnology, v. 18, n. 1, p. 116-122, JAN-MAR 2018. (15/26837-0, 12/08307-5, 13/08884-5)

ALVES-PEREIRA, ALESSANDRO; CLEMENT, CHARLES R.; PICANCO-RODRIGUES, DORIANE; VEASEY, ELIZABETH ANN; DEQUIGIOVANNI, GABRIEL; FERREYRA RAMOS, SANTIAGO LINORIO; PINHEIRO, JOSE BALDIN; DE SOUZA, ANETE PEREIRA; ZUCCHI, MARIA IMACULADA. A population genomics appraisal suggests independent dispersals for bitter and sweet manioc in Brazilian Amazonia. EVOLUTIONARY APPLICATIONS, v. 13, n. 2, OCT 2019. (12/08307-5, 18/00036-9, 13/08884-5, 13/00003-0)

ALVES-PEREIRA, ALESSANDRO; CLEMENT, CHARLES R.; PICANCO-RODRIGUES, DORIANE; VEASEY, ELIZABETH A.; DEQUIGIOVANNI, GABRIEL; RAMOS, SANTIAGO L. F.; PINHEIRO, JOSE B.; ZUCCHI, MARIA I.. Patterns of nuclear and chloroplast genetic diversity and structure of manioc along major Brazilian Amazonian rivers. ANNALS OF BOTANY, v. 121, n. 4, p. 625-639, MAR 2018. (13/00003-0, 12/08307-5, 13/11137-7, 13/08884-5)

ALVES-PEREIRA, ALESSANDRO; NOVELLO, MARIANA; DEQUIGIOVANNI, GABRIEL; PINHEIRO, JOSE BALDIN; BRANCALION, PEDRO H. S.; VEASEY, ELIZABETH ANN; CLEMENT, CHARLES R.; DE SOUZA, ANETE PEREIRA; ZUCCHI, MARIA IMACULADA. Genomic Diversity of Three Brazilian Native Food Crops Based on Double-Digest Restriction Site-Associated DNA Sequencing. TROPICAL PLANT BIOLOGY, v. 12, n. 4, p. 268-281, DEC 2019. (12/08307-5, 18/00036-9, 13/17354-0, 13/08884-5, 13/00003-0)

DEQUIGIOVANNI, G.; RAMOS, S. L. F.; ZUCCHI, M. I.; BAJAY, M. M.; PINHEIRO, J. B.; FABRI, E. G.; BRESSAN, E. A.; VEASEY, E. A.. Isolation and characterization of microsatellite loci for Bixa orellana, an important source of natural dyes. Genetics and Molecular Research, v. 13, n. 4, p. 9097-9102, 2014. (12/08307-5, 11/05243-3, 13/08884-5)

DEQUIGIOVANNI, GABRIEL; FERREYRA RAMOS, SANTIAGO LINORIO; ALVES-PEREIRA, ALESSANDRO; FABRI, ELIANE GOMES; PICANCO-RODRIGUES, DORIANE; CLEMENT, CHARLES ROLAND; GEPTS, PAUL; VEASEY, ELIZABETH ANN. Highly structured genetic diversity of Bixa orellana var. urucurana, the wild ancestor of annatto, in Brazilian Amazonia. PLoS One, v. 13, n. 6, JUN 6 2018. (15/26837-0, 12/08307-5, 16/05912-6, 13/08884-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

DEQUIGIOVANNI, Gabriel. Genetic structure, mating system and domestication of annatto (Bixa orellana L.) using molecular markers. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC) Piracicaba.