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Protein profile in Aspergillus nidulans recombinant strains overproducing heterologous enzymes

Grant number: 17/22663-2
Support type:Regular Research Grants - Publications - Scientific article
Duration: February 01, 2018 - July 31, 2018
Field of knowledge:Biological Sciences - Microbiology
Principal Investigator:André Ricardo de Lima Damasio
Grantee:André Ricardo de Lima Damasio
Home Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas, SP, Brazil
Associated research grant:12/20549-4 - Secretion of heterologous glycoproteins in Aspergillus: effect of glycosylation pattern in functional parameters of glycosyl hydrolases, AP.BIOEN.JP

Abstract

Filamentous fungi are robust cell factories and have been used for the production of large quantities of industrially relevant enzymes. However, the production levels of heterologous proteins still need to be improved. Therefore, this article aims to investigate the global proteome profiling of Aspergillus nidulans recombinant strains in order to understand the bottlenecks of heterologous enzymes production. About 250, 441 and 424 intracellular proteins were identified in the control strain Anid_pEXPYR, and in the recombinant strains Anid_AbfA and Anid_Cbhl, respectively. In this context, the most enriched processes in recombinant strains were energy pathway, amino acid metabolism, ribosome biogenesis, translation, endoplasmic reticulum and oxidative stress, and repression under secretion stress (RESS). The global protein profile of the recombinant strains Anid_AbfA and Anid_Cbhl were similar, although the latter strain secreted more recombinant enzyme than the former. These findings provide insights into the bottlenecks involved in the secretion of recombinant proteins in A. nidulans, as well as in regards to the rational manipulation of target genes for engineering fungal strains as microbial cell factories. (AU)