Development of serological assay for Zika virus IgG and IgM based on low coast ELISA

• Grant number: 16/08727-5
• Support Opportunities: Research Grants - Innovative Research in Small Business - PIPE
• Start date: February 01, 2017
• End date: September 30, 2019
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Agreement: FINEP - PIPE/PAPPE Grant
• Principal Investigator: Danielle Bruna Leal de Oliveira
• Grantee: Danielle Bruna Leal de Oliveira
• Company: Comércio, Importação e Exportação Bioscan Ltda
• Principal investigators: Edison Luiz Durigon ; Luis Carlos de Souza Ferreira
• Associated researchers: Roxane Maria Fontes Piazza ; Viviane Fongaro Botosso

Abstract

The present invention describes the identification, production and testing of a recombinant ZIKV antigen which allows the development of specific serological tests for those infected with the virus. Unlike tests available on the market, the present antigen detected specifically antibodies produced in ZIKV-infected patients even from people which were infected previously by other flavivirus such DENV, making this antigen suitable for use in endemic regions for DENV and other arboviruses. In addition, this antigen allowed the identification of ZIKV-infected people years after infection, in a simple, fast and inexpensive way. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

OLIVEIRA, DANIELLE B. L.; DURIGON, GIULIANA S.; MENDES, ERICA A.; LADNER, JASON T.; ANDREATA-SANTOS, ROBERT; ARAUJO, DANIELLE B.; BOTOSSO, VIVIANE F.; PAOLA, NICHOLAS D.; NETO, DANIEL F. L.; CUNHA, MARIELTON P.; et al. Persistence and Intra-Host Genetic Evolution of Zika Virus Infection in Symptomatic Adults: A Special View in the Male Reproductive System. Viruses-Basel, v. 10, n. 11, NOV 2018. (13/07467-1, 14/17766-9, 14/27228-4, 14/16333-1, 16/08727-5, 15/25643-7, 14/03911-7, 16/08204-2, 16/20045-7, 13/26942-2, 16/03605-9)

BARBOSA, CARLA M.; DI PAOLA, NICHOLAS; CUNHA, MARIELTON P.; RODRIGUES-JESUS, MONICA J.; ARAUJO, DANIELLE B.; SILVEIRA, VANESSA B.; LEAL, FABYANO B.; MESQUITA, FLAVIO S.; BOTOSSO, VIVIANE F.; ZANOTTO, PAOLO M. A.; et al. Yellow Fever Virus RNA in Urine and Semen of Convalescent Patient, Brazil. Emerging Infectious Diseases, v. 24, n. 1, p. 176-178, JAN 2018. (14/17766-9, 14/21141-4, 16/08727-5, 13/22136-1, 16/08204-2)

SLAVOV, SVETOSLAV NANEV; GUARAGNA MACHADO, RAFAEL RAHAL; FERREIRA, ALEXANDER RODRIGO; SOARES, CAMILA PEREIRA; ARAUJO, DANIELLE BASTOS; LEAL OLIVEIRA, DANIELLE BRUNA; COVAS, DIMAS TADEU; DURIGON, EDISON LUIZ; KASHIMA, SIMONE. Zika virus seroprevalence in blood donors from the Northeastern region of Sao Paulo State, Brazil, between 2015 and 2017. Journal of Infection, v. 80, n. 1, p. 111-115, JAN 2020. (17/23205-8, 16/20045-7, 16/15049-3, 16/08727-5, 14/50947-7)

CAMPOS, ANGELICA CRISTINE DE ALMEIDA; CICOLO, SOFIA; DE OLIVEIRA, CAIRO MONTEIRO; MOLINA, CAMILA VIEIRA; NAVAS-SUAREZ, PEDRO ENRIQUE; DOS SANTOS, THAIS POLTRONIERI; DA SILVEIRA, VANESSA BARBOSA; BARBOSA, CARLA MENEGUIN; BACCARIN, RAQUEL YVONNE ARANTES; DURIGON, EDISON LUIZ; et al. Potential outbreak by herpesvirus in equines: detection, clinical, and genetic analysis of equid gammaherpesvirus 2 (EHV-2). Brazilian Journal of Microbiology, v. N/A, p. 7-pg., 2023-01-27. (18/05346-6, 16/08727-5, 14/15090-8, 20/01487-4, 18/18867-4)

AROMOLARAN, ADEOLU; ARAUJO, KATIACI; LADINES-LIM, JOSEPH B.; NERY JR, NIVISON; ROSARIO, MATEUS S. DO; RASTELY JR, VALMIR N.; ARCHANJO, GRACINDA; DALTRO, DINA; CARVALHO, GUSTAVO BALTAZAR DA SILVEIRA; PIMENTEL, KLEBER; et al. Unequal burden of Zika-associated microcephaly among populations with public and private healthcare in Salvador, Brazil. INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, v. 120, p. 4-pg., 2022-07-01. (16/08727-5)