Development of healing pharmaceutical formulation containing bromelain extracted from industrial wastes

• Grant number: 16/03444-5
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2016
• End date: March 31, 2019
• Field of knowledge: Health Sciences - Pharmacy
• Principal Investigator: Priscila Gava Mazzola
• Grantee: Priscila Gava Mazzola
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• City of the host institution: Campinas
• Associated researchers: Janaína Artem Ataide ; Leticia Caramori Cefali

Abstract

Bromelain is a protease, obtained from different species from Bromeliaceae family, and it is present in the pineapple stem and fruit and is disposed in juice and jelly production. This biomolecule is known for its clinical and therapeutic applications, mainly the burn selective debridement, improvement of antibiotic and anti-inflammatory action, anti-thrombotic and fibrinolytic action. Its therapeutic potential is poorly explored due to its physical and chemical instability, which prevent to reach their full potential of action, once it easily denatures. Thereby, this projects aims bromelain encapsulation (micro and nano scale) using polymeric particles. the particulate systems are able to afford better stability, efficacy and safety for the encapsulated active and have attracted the pharmaceutical industry interest. After encapsulation process, the obtained particles will be characterized and will be evaluated its release profile, bromelain enzymatic activity, stability, in vitro pharmacological activity and toxicological profile. Then particles will be incorporated into semi-solid formulations for topical use. Those formulations will be evaluated according with pharmacopoeic requeriments. At this project end, it is intended to obtain a formulation containing bromelain, which attends the stability and therapeutic efficacy requirement for human use. (AU)

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ATAIDE, JANAINA ARTEM; GERIOS, ELOAH FAVERO; MAZZOLA, PRISCILA GAVA; SOUTO, ELIANA B.. Bromelain-loaded nanoparticles: A comprehensive review of the state of the art. ADVANCES IN COLLOID AND INTERFACE SCIENCE, v. 254, p. 48-55, APR 2018. (17/05333-9, 16/03444-5, 17/05275-9)

ATAIDE, JANAINA ARTEM; GERIOS, ELOAH FAVERO; CEFALI, LETICIA CARAMORI; FERNANDES, ANA RITA; TEIXEIRA, MARIA DO CEU; FERREIRA, NUNO R.; TAMBOURGI, ELIAS BASILE; JOZALA, ANGELA FAUSTINO; CHAUD, MARCO VINICIUS; OLIVEIRA-NASCIMENTO, LAURA; et al. Effect of Polysaccharide Sources on the Physicochemical Properties of Bromelain-Chitosan Nanoparticles. POLYMERS, v. 11, n. 10, OCT 2019. (17/05333-9, 16/03444-5, 17/05275-9)

CROISFELT, FERNANDA MACHADO; TUNDISI, LOUISE LACALENDOLA; ATAIDE, JANAINA ARTEM; SILVEIRA, EDGAR; TAMBOURGI, ELIAS BASILE; JOZALA, ANGELA FAUSTINO; BARBOSA SOUTO, ELIANA MARIA; MAZZOLA, PRISCILA GAVA. Modified-release topical hydrogels: a ten-year review. Journal of Materials Science, v. 54, n. 16, p. 10963-10983, AUG 2019. (16/03444-5)

ATAIDE, JANAINA ARTEM; CEFALI, LETICIA CARAMORI; CROISFELT, FERNANDA MACHADO; MARTINS SHIMOJO, ANDREA ARRUDA; OLIVEIRA-NASCIMENTO, LAURA; MAZZOLA, PRISCILA GAVA. Natural actives for wound healing: A review. Phytotherapy Research, v. 32, n. 9, p. 1664-1674, SEP 2018. (16/03444-5, 15/15068-5)

ATAIDE, JANAINA A.; ZANCHETTA, BEATRIZ; SANTOS, ERICA M.; FAVA, ANA LAURA M.; ALVES, THAIS F. R.; CEFALI, LETICIA C.; CHAUD, MARCO, V; OLIVEIRA-NASCIMENTO, LAURA; SOUTO, ELIANA B.; MAZZOLA, PRISCILA G.. Nanotechnology-Based Dressings for Wound Management. PHARMACEUTICALS, v. 15, n. 10, p. 16-pg., 2022-10-01. (15/15068-5, 17/05275-9, 18/07707-6, 16/03444-5, 20/11333-4)

ATAIDE, JANAINA ARTEM; GERALDES, DANILO COSTA; GERIOS, ELOAH FAVERO; BISSACO, FERNANDA MAZON; CEFALI, LETICIA CARAMORI; OLIVEIRA-NASCIMENTO, LAURA; MAZZOLA, PRISCILA GAVA. Freeze-dried chitosan nanoparticles to stabilize and deliver bromelain. JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY, v. 61, FEB 2021. (16/02282-1, 16/02287-3, 16/03444-5, 15/15068-5)

GIAROLA, LEILA REGINA; COCO, JULIA CEDRAN; SOUSA, ILZA MARIA DE OLIVEIRA; CEFAL, LETICIA CARAMORI; ATAIDE, JANAINA ARTEM; TAVARES, GUILHERME DINIZ; RODRIGUES, RODNEY ALEXANDRE FERREIRA; FOGLIO, MARY ANN; MAZZOLA, PRISCILA GAVA. Pterodon pubescens Benth (sucupira) microencapsulation influence on formulation stability outcome compared to non-encapsulated extract. JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY, v. 67, p. 13-pg., 2022-01-01. (18/07707-6, 16/03444-5, 15/09293-6, 18/06475-4, 13/06384-5)

ATAIDE, JANAINA ARTEM; CEFALI, LETICIA CARAMORI; FIGUEIREDO, MARIANA CECCHETTO; DE OLIVEIRA BRAGA, LUCIA ELAINE; TASCA GOIS RUIZ, ANA LUCIA; FOGLIO, MARY ANN; OLIVEIRA-NASCIMENTO, LAURA; MAZZOLA, PRISCILA GAVA. In vitro performance of free and encapsulated bromelain. SCIENTIFIC REPORTS, v. 11, n. 1, MAY 13 2021. (16/03444-5, 15/15068-5)

COCO, JULIA CEDRAN; TUNDISI, LOUISE LACALENDOLA; DOS SANTOS, ERICA MENDES; MASQUETTI FAVA, ANA LAURA; ALVES, THAIS FRANCINE; ATAIDE, JANAINA ARTEM; CHAUD, MARCO VINICIUS; MAZZOLA, PRISCILA GAVA. PVA-CO-AAM and peg-co-aam hydrogels as bromelain carriers. JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY, v. 63, JUN 2021. (15/15068-5, 16/03444-5)

CROISFELT, FERNANDA MACHADO; ATAIDE, JANAINA ARTEM; TUNDISI, LOUISE LACALENDOLA; CEFALI, LETICIA CARAMORI; REBELO, MARCIA DE ARAUJO; DAVILA SANCHEZ, JOSE LUIS; DA COSTA, TAIS GERMANO; LIMA, RENATA; JOZALA, ANGELA FAUSTINO; CHAUD, MARCO VINICIUS; et al. Characterization of PNIPAAm-co-AAm hydrogels for modified release of bromelain. EUROPEAN POLYMER JOURNAL, v. 105, p. 48-54, AUG 2018. (16/03444-5)

Filed patent(s) as a result of this research project