Advanced search
Start date
Betweenand

N-glycosylation and enzymes secretion in filamentous fungi

Grant number: 17/22669-0
Support type:Program for Research on Bioenergy (BIOEN) - Regular Program Grants
Duration: August 01, 2018 - July 31, 2020
Field of knowledge:Biological Sciences - Microbiology
Principal Investigator:André Ricardo de Lima Damasio
Grantee:André Ricardo de Lima Damasio
Home Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas, SP, Brazil

Abstract

Considering the entire conversion of plant biomass into bioproducts, such as the ethanol obtained from sugarcane bagasse (E2G), the enzymes make up a significant percentage of overall cellulosic ethanol production costs, ranging from 28% for off-site production to 22% for on-site and 10% for integrated enzyme production. The global market for industrial enzymes is expected to reach nearly $6.2 billion by 2020. About 12 major producers and 400 minor suppliers satisfy the world enzyme demand. Three top enzyme companies, i.e. Denmark-based Novozymes, US-based DuPont and Switzerland-based Roche, produce 75% of the total enzymes. The market is highly competitive, has small profit margins and is technologically intensive. Brazil is back behind and currently, there are no companies developing national technology for enzyme production. Here we propose to manipulate genes related to N-glycosylation in A. nidulans to deeply understand how this posttranslational modification can influence the yields of enzymes secretion. Our hypothesis is based on previous results from our group that showed it is possible to improve a target enzyme secretion by N-glycosylation manipulation. (AU)